Supplementary Materials for Guigó et al (2003)
PNAS, 100(3):1140-1145 [ Abstract ]
  IMIM * UPF * CRG * GRIB HOME DATASETS Mouse/Human GP Chr8
   
CHROMOSOME 8 RT-PCR SUBMITTED GENES SUMMARY

GENEVA
CODE
GENE
ID
GENE
LOCATION
CDS LEN
/ #EXONS
RTPCR
INTRON
 
BR
 
HE
 
KI
 
TH
 
LI
 
ST
 
MU
 
LU
 
TE
 
SK
 
EY
 
OV
SEQ
CNF
SEQ
WRG
DIF
SPL
6E5 SGP.chr8_195  13951923 - 13959894 REV 465bp / 3 2 1 1 1 1 1 1 1 1 1 1 1 1 1 0 0
4G6 TS.chr8.14.033  13951926 - 13959424 REV 495bp / 3 2 1 1 1 1 1 1 0 1 1 1 1 1 1 0 0
1H2 SGP.chr8_320  24675205 - 24707607 REV 1251bp / 11 8 1 1 1 1 1 1 1 1 1 1 1 1 1 0 0
7B6 SGP.chr8_345  25590700 - 25626547 REV 309bp / 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
11B5 SGP.chr8_404  28854618 - 28901314 REV 498bp / 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
9D1 TS.chr8.32.015  31977485 - 31979790 REV 600bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
6E6 SGP.chr8_673  48222620 - 48266796 FWD 729bp / 5 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
1H3 SGP.chr8_813  60854781 - 60955001 REV 1569bp / 10 9 0 1 0 1 1 0 1 1 1 0 1 0 1 0 0
4H1 TS.chr8.62.002  61042157 - 61048023 REV 1071bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
9F5 SGP.chr8_830  63457105 - 63529880 REV 381bp / 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
4H2 TS.chr8.72.034  71948091 - 71951324 REV 225bp / 5 3 1 1 1 1 1 1 1 1 1 1 1 1 1 0 0
6E2 SGP.chr8_1103  78297832 - 78336552 REV 570bp / 4 2 0 0 1 0 0 0 0 1 1 1 0 0 0 1 0
1H1 SGP.chr8_1151  82647203 - 82766018 FWD 897bp / 8 5 1 1 0 1 0 0 1 1 0 0 0 1 1 0 0
9E1 TS.chr8.83.010  82723249 - 82728488 FWD 465bp / 3 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
12E5 TS.chr8.84.030  83734835 - 83736695 REV 423bp / 2 1 1 0 0 1 0 0 0 1 0 0 0 1 1 0 0
8E2 SGP.chr8_1275  87784201 - 87798866 REV 450bp / 4 2 1 1 1 1 1 1 1 1 1 1 1 1 0 1 0
6E3 SGP.chr8_1347  90254550 - 90266641 FWD 270bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
8E3 SGP.chr8_1383  91562353 - 91583034 FWD 333bp / 2 1 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0
8E4 SGP.chr8_1392  91811380 - 91845929 FWD 570bp / 4 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0
9E5 SGP.chr8_1611  103469631 - 103506859 REV 381bp / 3 2 0 0 0 0 0 0 0 1 1 0 0 0 0 1 0
4G5 TS.chr8.105.007  104237475 - 104248176 REV 510bp / 4 2 1 1 1 1 1 1 1 1 1 1 1 1 1 0 0
6E4 SGP.chr8_1758  107782374 - 107790368 REV 354bp / 4 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
9C1 TS.chr8.115.009  114419142 - 114421259 REV 504bp / 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
GENEVA
CODE
GENE
ID
GENE
LOCATION
CDS LEN
/ #EXONS
RTPCR
INTRON
BR HE KI TH LI ST MU LU TE SK EY OV SEQ
CNF
SEQ
WRG
DIF
SPL
 

TABLE COLUMNS DESCRIPTION

Here we describe what does contain each column in the previous table:

  • Geneva identifier: corresponds to an alphanumeric code for each single gene set of experiments.
  • Predicted gene identifier: the name of the given gene prediction as provided by the software. Both, this column and the previous one, are linked to a single browser page for each gene prediction that contains further information about it.
  • Gene location: the forward coordinates along the chromosome and the corresponding gene strand.
  • Length of the transcript and the number of exons.
  • The selected intron number.
  • Results on tissues: RT-PCR success on each of the mouse tissues tested in this experiment ("green" color meaning success and "red" color for failure). The tissues were: brain (BR), heart (HE), kidney (KI), thymus (TH), liver (LI), stomach (ST), muscle (MU), lung (LU), testis (TE), skin (SK), eye (EY) and ovary (OV).
  • Three extra columns summarizing RT-PCR results:
    • SEQ CNF : RT-PCR succeeded and the sequence of the predicted gene was confirmed.
    • SEQ WRG : the PCR product that was sequenced does not correspond to the predicted one (unspecific amplification).
    • DIF SPL : the RT-PCR determined a different splice site (i.e. an extra exon within the submitted intron).
    Real Positive Genes are those, and only those, having "1" in the "SEQ CNF" column.

 
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